VHC Vitamin-D Test PrincipleVHC Vitamin-D Test Principle

The Vitality Health Check quantitative Vitamin D test (VHC VItamin-D) utilizes the principle of immuno chromatography, a unique two-site “Sandwich” immunoassay on a membrane. The test employs an exclusive pair of anti-25-0H-Vitamin-D monoclonal antibodies; one conjugated with colloidal gold and another one immobilized on the solid phase. This will selectively detect Vitamin D with a high degree of sensitivity and specificity.

As the test sample flows through the membrane assembly within the test device, the colored anti-25-0H-Vitamin-D-colloidal gold conjugate complexes with 25-0H-Vitamin D from the sample. This complex moves further on the membrane by the capillary action to the test region (T) where it is immobilized by another anti-25-0H-Vitamin-D coated on the membrane, leading to formation of a pink / purple colored band, which confirms a positive test results. The intensity of colored band in the test line region is 25-0H-Vitamin-D concentration-dependent, higher the concentration of 25-0H-Vitamin-D in the tested sample, the stronger the colored band is. A control line is present in the test window to work as procedural control. This colored band should always appear on the control line region (C) if the test device is stored in good condition and the test is performed appropriately.

HOW TO RUN THE TESTVHC Vitamin-D Test Principle
A small sample of 10 microliter capillary blood (from finger-prick) is required to run the test.
The sample is pre mixed with the sample buffer in our UniSampler, a special sample collection and mixing device and then dropped on the sample pad of the VHC Vitamin-D lateral flow test

After 15 minutes the result is automatically read and quantified by the Vitality Health Check Health Reader (VHC-Reader).

The  full “Instructions for use” and other documents for professional users of the VHC Vitamin-D test can be found in the SERVICE section of this website.

Declaration of conformity (DoC) – CE marking Europe